Purification of a peroxidase from Solanum melongena fruit juice.

نویسندگان

  • S K Vernwal
  • R S S Yadav
  • K D S Yadav
چکیده

Solanum melongena fruit juice contains peroxidase activity of the order of 0.125 IU/mL. A method for the 11-fold purification of the enzyme was developed. The Km values of the peroxidase for the substrates guaiacol and hydrogen peroxide were 6.5 mM and 0.33 mM, respectively. The pH and temperature optima were 5.5 and 84 degrees C, respectively using guaiacol as the substrate. Sodium azide and phenyl hydrazine inhibited the enzyme competitively.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Is Root Catalase a Bifunctional Catalase-Peroxidase?

Plant catalases exhibit spatial and temporal distribution of their activity. Moreover, except from the typical monofunctional catalase, a bifunctional catalase-peroxidase has been reported. The aim of this study was to investigate whether the leaf and root catalases from six different plant species (Lactuca sativa, Cichorium endivia, Apium graveolens, Petroselinum crispum, Lycopersicon esculent...

متن کامل

Effect of grafting on growth, performance and yield of aubergine (Solanum melongena L.) in greenhouse and open-field

Seedlings of aubergine (Solanum melongena L.) cv. ‘Rima’ were used as scion (R) and control (non-grafted) and rootstock (self-grafted) (RR), while two hybrid tomatoes ‘Heman’ (Lycopersicon hirsutm) and ‘Primavera’ (Lycopersicon esculentum) were used as rootstocks. Grafted and non-grafted plants were grown in the greenhouse and in the open-field. Grafted plants Rima and Heman (RH) were more vigo...

متن کامل

Study of Variation of Yield and Morphological Traits of Some Local Varieties of Iran’s Eggplant (Solanum melongena L.)

Eggplant is a crop that has been cultivated for a long time in different areas of Iran. Nevertheless, no sufficient studies have been done on variation of morphological traits and yield of this crop in Iran, and it has not been well-attended in breeding programs. Therefore, to study genetic diversity in collected local varieties, the experiment was conducted in a Randomized Complete Block Desig...

متن کامل

Genetic Diversity, Population Structure, and Resistance to Phytophthora capsici of a Worldwide Collection of Eggplant Germplasm

Eggplant (Solanum melongena L.) is an important solanaceous crop with high phenotypic diversity and moderate genotypic diversity. Ninety-nine genotypes of eggplant germplasm (species (S. melongena, S. incanum, S. linnaeanum and S. gilo), landraces and heirloom cultivars) from 32 countries and five continents were evaluated for genetic diversity, population structure, fruit shape, and disease re...

متن کامل

Soft Rot of Eggplant (Solanum melongena) Caused by Choanephora cucurbitarum in Korea

In April 2002 and 2003, soft rot on fruit of eggplant (Solanum melongena) caused by Choanephora cucurbitarum was observed in the experimental fields at Gyeongnam Agricultural Research and Extension Services in Korea. The disease began with water-soaking and dark-green lesions, and then the infected tissues were rapidly rotten. Sporangium was subglobose in shape and sized 40~130 µm. Monosporous ...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Indian journal of biochemistry & biophysics

دوره 43 4  شماره 

صفحات  -

تاریخ انتشار 2006